Inhibitor-Tolerant RT-qPCR Mix:
Develop an Extraction-Free Test Using Crude Sputum Saliva or Stool Samples

In an RT-qPCR test, the RNA extraction step is a time limiting step and RNA can be lost during the extraction process reducing the performance of the assay. However, extraction is typically required to remove inhibitors which would otherwise impact and assay’s sensitivity and accuracy.

In order to address this challenge, Meridian has developed an Inhibitor-Tolerant RT-qPCR Mix capable of delivering sensitive multiplex detection, even in the presence of difficult inhibitors found in sputum, saliva and stool specimens. This new mix offers a novel alternative to nucleic acids extraction, saving both time and labor which are vitally important in viral screening assays that require rapid detection for infection control.

The formulation is supplied as a concentrated (4x) mix in order to maximize sample input volume and aiding in low copy number detection. In addition, the one-tube format of Meridian’s Inhibitor-Tolerant RT-qPCR Mix simplifies the optimization and reaction set-up.

Inhibitor-Tolerant RT-qPCR Mix (MDX016)

  • Designed for crude samples without the need for complex or time consuming extraction
  • 4x concentration master mix allows for greater sample volume
  • All-in-one simple to use one-step mix. Only add primers, probes and clinical samples
  • Suitable for single and multiplex detection of RNA and DNA pathogens

  • Inhibitor Tolerant RT-qPCR Mix exhibits a high tolerance to PCR inhibitors from clinical samples

Amplification profile of inactivated influenza virus spiked into samples containing 5% sputum or no sputum. The data illustrates that the performance of Inhibitor-Tolerant RT-qPCR Mix (MDX016) in the presence of 5% artificial sputum (red) is the same Fast One-Step RT-qPCR Mix (MDX032) with no sputum (blue). In contrast, the sensitivity and performance of Fast One-Step RT-qPCR Mix (MDX032) significantly decreases in the presence of 5% artificial sputum (black) compared to no sputum or compared to Inhibitor-Tolerant RT-qPCR Mix (MDX016) with sputum.